Abstract
Phytochemicals extraction for medicinal usefulness depends on the nature of solvents used. The rinsed leaves of fluted pumpkin were cut into smaller pieces for easy air drying, ground and sieved to give 40 mm mesh size powder. The focus of this research is to separately use five solvents (acetone, chloroform, water, methanol and ethyl acetate) to extract phytochemicals from fluted pumpkin leaves, and investigate the extractive values of each solvent as well as qualitatively screened each solvent extract for nine phytochemicals (flavonoid, carotenoid, phenol, oxalate, tannin, saponin, alkaloid, phytate and ascorbic acid) using standard procedures. The leaves of fluted pumpkin were obtained, rinsed, cut into smaller pieces, air-dried, ground into powdery sample, and sieved with 40 mm mesh size. 20 g of powdered fluted pumpkin leaf was separately extracted using 200 mL of five different solvents for 72 h. The extractive values (%) in methanol, water, acetone, ethyl acetate and chloroform were 9.95±0.78, 8.80±0.00, 6.36±0.27, 5.84±0.22 and 5.71±0.68 respectively. The extractive values of the fluted pumpkin leaf were highest in methanol and water but least in chloroform and ethyl acetate. The raw fluted pumpkin leaves contained seven out of nine phytochemicals screened for and this amounted to 77.8% detectable phytochemicals. Methanol and water extracts contained 77.8% detectable phytochemicals as contained in the raw fluted pumpkin leaves. Acetone, ethyl acetate and chloroform extracts of fluted pumpkin leaves had 66.7%, 55.6% and 44.4% detectable phytochemicals accordingly. Methanol, water and acetone were better solvents in extracting bioactive ingredients from fluted pumpkin leaves.
Presenters
Jacob Olalekan ArawandeReader, Science Laboratory Technology, University of Medical Sciences, Ondo, Nigeria Akinyinka Akinnusotu
University of Medical Sciences, Ondo City, Nigeria C.O. Ayodele
University of Medical Sciences, Ondo City, Nigeria
Details
Presentation Type
Paper Presentation in a Themed Session
Theme
Food Production and Sustainability
KEYWORDS
EXTRACTION, EXTRACTIVE VALUE, FLUTED PUMPKIN LEAF, PHYTOCHEMICALS, SOLVENTS
